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KMID : 1098420040120060478
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Korean Journal of Medicinal Crop Science
2004 Volume.12 No. 6 p.478 ~ p.482
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Production of Gomisin J from Suspension Cultured Cells of Schisandra chinensis Baillon in Airlift-type Bioreactor
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Hwang Sung-Jin
Pyo Byoung-Sik
Hwang Baik
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Abstract
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Suspension culture of Schisandra chinensis for production of gomisin J was perfomed in bioreactor. The inoculum size and initial sucrose concentration had significant effect on the cell growth and gomisin J accumulation. The maximum dry cell weight (DCW;;43.5;g/{ell}) and gomisin J content (0.71;{times};10^{-3};{mu}g/g;DCW) were obtained at inoculum size of 100 g fresh cell weight (FCW) per liter and MB5 medium containing 6% sucrose after 8 weeks of culture. The effect of oxygen supply on the cell growth and gomisin J accumulation was also investigated in an airlift-type bioreactor. The optimal cell growth and gomisin J content was obtained under 0.5 vvm. The productivity of gomisin J was 0.7 fold in bioreactor culture lower than that obtained in a flask cultivation.
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KEYWORD
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Schisandra chinensis, suspension cell cultures, gomisin J, airlift-type bioreactor
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